Molecular Characterization of UpaB and UpaC, Two New Autotransporter Proteins of Uropathogenic Escherichia coli CFT073
Identifieur interne : 005314 ( Main/Exploration ); précédent : 005313; suivant : 005315Molecular Characterization of UpaB and UpaC, Two New Autotransporter Proteins of Uropathogenic Escherichia coli CFT073
Auteurs : Luke P. Allsopp [Australie] ; Christophe Beloin [France] ; Glen C. Ulett [Australie] ; Jaione Valle [France] ; Makrina Totsika [Australie] ; Orla Sherlock [Australie] ; Jean-Marc Ghigo [France] ; Mark A. Schembri [Australie]Source :
- Infection and Immunity [ 0019-9567 ] ; 2012.
Abstract
Uropathogenic
Url:
DOI: 10.1128/IAI.05322-11
PubMed: 21930758
PubMed Central: 3255655
Affiliations:
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Le document en format XML
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CFT073</title>
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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Molecular Characterization of UpaB and UpaC, Two New Autotransporter Proteins of Uropathogenic <named-content content-type="genus-species">Escherichia coli</named-content>
CFT073</title>
<author><name sortKey="Allsopp, Luke P" sort="Allsopp, Luke P" uniqKey="Allsopp L" first="Luke P." last="Allsopp">Luke P. Allsopp</name>
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<author><name sortKey="Beloin, Christophe" sort="Beloin, Christophe" uniqKey="Beloin C" first="Christophe" last="Beloin">Christophe Beloin</name>
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<country xml:lang="fr">France</country>
<wicri:regionArea>Institut Pasteur, Unité de Génétique des Biofilms, Département de Microbiologie, Paris</wicri:regionArea>
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<author><name sortKey="Ulett, Glen C" sort="Ulett, Glen C" uniqKey="Ulett G" first="Glen C." last="Ulett">Glen C. Ulett</name>
<affiliation wicri:level="1"><nlm:aff id="aff4">Centre for Medicine and Oral Health Campus, Griffith University, Southport, QLD, Australia</nlm:aff>
<country xml:lang="fr">Australie</country>
<wicri:regionArea>Centre for Medicine and Oral Health Campus, Griffith University, Southport, QLD</wicri:regionArea>
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<author><name sortKey="Valle, Jaione" sort="Valle, Jaione" uniqKey="Valle J" first="Jaione" last="Valle">Jaione Valle</name>
<affiliation wicri:level="3"><nlm:aff id="aff2">Institut Pasteur, Unité de Génétique des Biofilms, Département de Microbiologie, Paris, France</nlm:aff>
<country xml:lang="fr">France</country>
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<placeName><region type="region">Île-de-France</region>
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<settlement type="city">Paris</settlement>
</placeName>
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<author><name sortKey="Totsika, Makrina" sort="Totsika, Makrina" uniqKey="Totsika M" first="Makrina" last="Totsika">Makrina Totsika</name>
<affiliation wicri:level="1"><nlm:aff id="aff1">Australian Infectious Disease Research Centre, School of Chemistry and Molecular Biosciences, University of Queensland, Brisbane, QLD, Australia</nlm:aff>
<country xml:lang="fr">Australie</country>
<wicri:regionArea>Australian Infectious Disease Research Centre, School of Chemistry and Molecular Biosciences, University of Queensland, Brisbane, QLD</wicri:regionArea>
<wicri:noRegion>QLD</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Sherlock, Orla" sort="Sherlock, Orla" uniqKey="Sherlock O" first="Orla" last="Sherlock">Orla Sherlock</name>
<affiliation wicri:level="1"><nlm:aff id="aff1">Australian Infectious Disease Research Centre, School of Chemistry and Molecular Biosciences, University of Queensland, Brisbane, QLD, Australia</nlm:aff>
<country xml:lang="fr">Australie</country>
<wicri:regionArea>Australian Infectious Disease Research Centre, School of Chemistry and Molecular Biosciences, University of Queensland, Brisbane, QLD</wicri:regionArea>
<wicri:noRegion>QLD</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Ghigo, Jean Marc" sort="Ghigo, Jean Marc" uniqKey="Ghigo J" first="Jean-Marc" last="Ghigo">Jean-Marc Ghigo</name>
<affiliation wicri:level="3"><nlm:aff id="aff2">Institut Pasteur, Unité de Génétique des Biofilms, Département de Microbiologie, Paris, France</nlm:aff>
<country xml:lang="fr">France</country>
<wicri:regionArea>Institut Pasteur, Unité de Génétique des Biofilms, Département de Microbiologie, Paris</wicri:regionArea>
<placeName><region type="region">Île-de-France</region>
<region type="old region">Île-de-France</region>
<settlement type="city">Paris</settlement>
</placeName>
</affiliation>
<affiliation wicri:level="3"><nlm:aff id="aff3">CNRS, URA2172, Paris, France</nlm:aff>
<country xml:lang="fr">France</country>
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<placeName><region type="region">Île-de-France</region>
<region type="old region">Île-de-France</region>
<settlement type="city">Paris</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Schembri, Mark A" sort="Schembri, Mark A" uniqKey="Schembri M" first="Mark A." last="Schembri">Mark A. Schembri</name>
<affiliation wicri:level="1"><nlm:aff id="aff1">Australian Infectious Disease Research Centre, School of Chemistry and Molecular Biosciences, University of Queensland, Brisbane, QLD, Australia</nlm:aff>
<country xml:lang="fr">Australie</country>
<wicri:regionArea>Australian Infectious Disease Research Centre, School of Chemistry and Molecular Biosciences, University of Queensland, Brisbane, QLD</wicri:regionArea>
<wicri:noRegion>QLD</wicri:noRegion>
</affiliation>
</author>
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<series><title level="j">Infection and Immunity</title>
<idno type="ISSN">0019-9567</idno>
<idno type="eISSN">1098-5522</idno>
<imprint><date when="2012">2012</date>
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<front><div type="abstract" xml:lang="en"><p>Uropathogenic <named-content content-type="genus-species">Escherichia coli</named-content>
(UPEC) is the primary cause of urinary tract infection (UTI) in the developed world. The major factors associated with virulence of UPEC are fimbrial adhesins, which mediate specific attachment to host receptors and trigger innate host responses. Another group of adhesins is represented by the autotransporter (AT) subgroup of proteins. The genome-sequenced prototype UPEC strain CFT073 contains 11 putative AT-encoding genes. In this study, we have performed a detailed molecular characterization of two closely related AT adhesins from CFT073: UpaB (c0426) and UpaC (c0478). PCR screening revealed that the <italic>upaB</italic>
and <italic>upaC</italic>
AT-encoding genes are common in <named-content content-type="genus-species">E. coli</named-content>
. The <italic>upaB</italic>
and <italic>upaC</italic>
genes were cloned and characterized in a recombinant <named-content content-type="genus-species">E. coli</named-content>
K-12 strain background. This revealed that they encode proteins located at the cell surface but possess different functional properties: UpaB mediates adherence to several ECM proteins, while UpaC expression is associated with increased biofilm formation. In CFT073, <italic>upaB</italic>
is expressed while <italic>upaC</italic>
is transcriptionally repressed by the global regulator H-NS. In competitive colonization experiments employing the mouse UTI model, CFT073 significantly outcompeted its <italic>upaB</italic>
(but not <italic>upaC</italic>
) isogenic mutant strain in the bladder. This attenuated phenotype was also observed in single-challenge experiments, where deletion of the <italic>upaB</italic>
gene in CFT073 significantly reduced early colonization of the bladder.</p>
</div>
</front>
</TEI>
<affiliations><list><country><li>Australie</li>
<li>France</li>
</country>
<region><li>Île-de-France</li>
</region>
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</settlement>
</list>
<tree><country name="Australie"><noRegion><name sortKey="Allsopp, Luke P" sort="Allsopp, Luke P" uniqKey="Allsopp L" first="Luke P." last="Allsopp">Luke P. Allsopp</name>
</noRegion>
<name sortKey="Schembri, Mark A" sort="Schembri, Mark A" uniqKey="Schembri M" first="Mark A." last="Schembri">Mark A. Schembri</name>
<name sortKey="Sherlock, Orla" sort="Sherlock, Orla" uniqKey="Sherlock O" first="Orla" last="Sherlock">Orla Sherlock</name>
<name sortKey="Totsika, Makrina" sort="Totsika, Makrina" uniqKey="Totsika M" first="Makrina" last="Totsika">Makrina Totsika</name>
<name sortKey="Ulett, Glen C" sort="Ulett, Glen C" uniqKey="Ulett G" first="Glen C." last="Ulett">Glen C. Ulett</name>
</country>
<country name="France"><region name="Île-de-France"><name sortKey="Beloin, Christophe" sort="Beloin, Christophe" uniqKey="Beloin C" first="Christophe" last="Beloin">Christophe Beloin</name>
</region>
<name sortKey="Beloin, Christophe" sort="Beloin, Christophe" uniqKey="Beloin C" first="Christophe" last="Beloin">Christophe Beloin</name>
<name sortKey="Ghigo, Jean Marc" sort="Ghigo, Jean Marc" uniqKey="Ghigo J" first="Jean-Marc" last="Ghigo">Jean-Marc Ghigo</name>
<name sortKey="Ghigo, Jean Marc" sort="Ghigo, Jean Marc" uniqKey="Ghigo J" first="Jean-Marc" last="Ghigo">Jean-Marc Ghigo</name>
<name sortKey="Valle, Jaione" sort="Valle, Jaione" uniqKey="Valle J" first="Jaione" last="Valle">Jaione Valle</name>
<name sortKey="Valle, Jaione" sort="Valle, Jaione" uniqKey="Valle J" first="Jaione" last="Valle">Jaione Valle</name>
</country>
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